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Why measure cell health

WEBThere are three main reasons why you may want to assess aspects of general cellular behavior: 1. You are investigating the mechanisms involved. Understanding normal, …

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URL: https://www.abcam.com/en-mc/technical-resources/guides/cell-health/why-measure-cell-health

Immunology and infectious diseases Abcam

WEBImmunology . Ground-breaking discoveries in immunology are changing how we understand the immune system and its impact on human health. New therapies …

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Cell proliferation: a complete guide Abcam

WEBCell proliferation can be used to assess normal cell health, to measure responses to toxic insult, or as a prognostic and diagnostic tool in several cancers. The available markers …

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Cell viability, cytotoxicity, and apoptosis assay guide Abcam

WEBCell viability assay guide. Including MTT, MTS, resazurin, and similar assays, mitochondrial membrane potential-dependent dyes, esterase cleaved dyes, ATP and ADP assays, and …

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Flow cytometry introduction abcam

WEBFlow cytometry is a widely used, laser-based method for analyzing the expression of cell surface and intracellular molecules. Discover more with our introduction to flow …

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Studying hallmarks of cancer abcam

WEBHere we review the latest version of the framework and provide the relevant markers and tools to study these important hallmarks of cancer. Core hallmarks of cancer. Genome …

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Cell cycle analysis with flow cytometry & propidium iodide Abcam

WEBCell cycle analysis by quantitation of DNA content was one of the earliest applications of flow cytometry. The DNA of mammalian, yeast, plant or bacterial cells can be stained by …

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Mitochondrial assays: biogenesis, toxicity, apoptosis Abcam

WEBMost popular Abcam mitochondrial membrane dye assay. Ex/Em 549/575 nm. Washed out after fixation. JC-1 (Ex/Em 530/530–570) and JC-10 (Ex/Em 590/520–570) form red …

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Antibody discovery and development services abcam

WEBOur antibody services include: 1. Antibody discovery. We have experience in challenging targets such as post-translationally modified proteins and small molecules, …

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Required controls for ELISA abcam

WEBPositive control. Use either an endogenous soluble sample known to contain the protein you are detecting or a purified protein or peptide known to contain the immunogen sequence …

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Annexin V staining assay protocol for apoptosis Abcam

WEBMethod. 1. Cell incubation with Annexin V-FITC. 1.1 Induce apoptosis via the desired method. 1.2 Collect 1–5 x 10 5 cells by centrifugation. 1.3 Resuspend cells in 500 µL of …

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Histone modifications Abcam

WEBAcetylation, methylation, phosphorylation, and ubiquitylation are the most well-understood, while GlcNAcylation, citrullination, crotonylation, sumoylation, and isomerization are …

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Health Assay Kit Lysosomal Metabolic

WEBRemove by aspiration. 6.1.5 Prepare staining medium: Add 10 µL of DMSO to one unit of LysoLiveTMEsterGreenTM. Dilute this solution 1:1000 in growth medium. (see Note (1)). …

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DAB staining principles, kits, and protocols Abcam

WEBDAB (3,3′-Diaminobenzidine) is a derivative of benzene. It is most often used in immunohistochemical (IHC) staining as a chromogen. It is also used in in situ …

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BrdU staining and BrdU assay protocol Abcam

WEBBrdU staining and BrdU assay protocol. BrdU (Bromodeoxyuridine / 5-bromo-2'-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to …

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MTT assay protocol Abcam

WEBWe recommend using a 5 mg/mL solution in PBS. Mix by vortexing or sonication. Filter sterilize solution after adding MTT. Store MTT solution at -20°C (stable for at least 6 …

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BioVision is now part of Abcam Abcam

WEBYou can be confident that this is the same product because the product overview section of our datasheet will reference the BioVision catalog number you searched. For example, if …

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Recombinant Anti-Bcl-2 antibody [NOR 235J] (ab241548)

WEBThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]. This product is a …

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2-NBDG Glucose Uptake Assay Kit (ab235976) Abcam

WEBThe kit uses 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. The 2-NBDG assay kit is suitable for …

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